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human gingival fibroblast cell line hgf 1  (ATCC)


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    ATCC human gingival fibroblast cell line hgf 1
    Human Gingival Fibroblast Cell Line Hgf 1, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1502 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 1502 article reviews
    human gingival fibroblast cell line hgf 1 - by Bioz Stars, 2026-06
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    ( a ) Boxplot of MTT assay absorbance (570 nm) after 24 h direct contact of human gingival <t>fibroblasts</t> with the tested denture base materials ( n = 9). ** p < 0.05, *** p < 0.005. ( b ) Mean MTT assay absorbance values (570 nm) of human gingival fibroblasts after 24 h of direct contact with the tested denture base materials.
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    ( a ) Boxplot of MTT assay absorbance (570 nm) after 24 h direct contact of human gingival fibroblasts with the tested denture base materials ( n = 9). ** p < 0.05, *** p < 0.005. ( b ) Mean MTT assay absorbance values (570 nm) of human gingival fibroblasts after 24 h of direct contact with the tested denture base materials.

    Journal: Journal of Functional Biomaterials

    Article Title: Evaluation of Post-Processing Time’s Influence on Biocompatibility of 3D-Printed Denture Base Resins

    doi: 10.3390/jfb17040188

    Figure Lengend Snippet: ( a ) Boxplot of MTT assay absorbance (570 nm) after 24 h direct contact of human gingival fibroblasts with the tested denture base materials ( n = 9). ** p < 0.05, *** p < 0.005. ( b ) Mean MTT assay absorbance values (570 nm) of human gingival fibroblasts after 24 h of direct contact with the tested denture base materials.

    Article Snippet: The commercially available human gingival fibroblasts (hGF) cell line (CLS Cell Lines Service GmbH, Eppelheim, Germany; catalog number 300703) was used for this study.

    Techniques: MTT Assay

    ( a ) Boxplot of MTT assay absorbance (570 nm) after 24 h of cell exposure to material eluates ( n = 9). ** p < 0.05, *** p < 0.005. ( b ) Mean MTT assay absorbance values (570 nm) of human gingival fibroblasts after 24 h of exposure to material eluates.

    Journal: Journal of Functional Biomaterials

    Article Title: Evaluation of Post-Processing Time’s Influence on Biocompatibility of 3D-Printed Denture Base Resins

    doi: 10.3390/jfb17040188

    Figure Lengend Snippet: ( a ) Boxplot of MTT assay absorbance (570 nm) after 24 h of cell exposure to material eluates ( n = 9). ** p < 0.05, *** p < 0.005. ( b ) Mean MTT assay absorbance values (570 nm) of human gingival fibroblasts after 24 h of exposure to material eluates.

    Article Snippet: The commercially available human gingival fibroblasts (hGF) cell line (CLS Cell Lines Service GmbH, Eppelheim, Germany; catalog number 300703) was used for this study.

    Techniques: MTT Assay

    ( a ) Boxplot of nitric oxide (NO) production, expressed as absorbance at 540 nm, in human gingival fibroblasts after 24 h of direct contact with the tested denture base materials ( n = 9). ( b ) Mean Griess assay absorbance values (540 nm) of human gingival fibroblasts after 24 h of direct contact with the tested denture base materials.

    Journal: Journal of Functional Biomaterials

    Article Title: Evaluation of Post-Processing Time’s Influence on Biocompatibility of 3D-Printed Denture Base Resins

    doi: 10.3390/jfb17040188

    Figure Lengend Snippet: ( a ) Boxplot of nitric oxide (NO) production, expressed as absorbance at 540 nm, in human gingival fibroblasts after 24 h of direct contact with the tested denture base materials ( n = 9). ( b ) Mean Griess assay absorbance values (540 nm) of human gingival fibroblasts after 24 h of direct contact with the tested denture base materials.

    Article Snippet: The commercially available human gingival fibroblasts (hGF) cell line (CLS Cell Lines Service GmbH, Eppelheim, Germany; catalog number 300703) was used for this study.

    Techniques: Griess Assay

    ( a ) Boxplot of nitric oxide (NO) production in human gingival fibroblasts after 24 h exposure to material eluates ( n = 9). ( b ) Mean Griess assay absorbance values of human gingival fibroblasts after 24 h of exposure to material eluates.

    Journal: Journal of Functional Biomaterials

    Article Title: Evaluation of Post-Processing Time’s Influence on Biocompatibility of 3D-Printed Denture Base Resins

    doi: 10.3390/jfb17040188

    Figure Lengend Snippet: ( a ) Boxplot of nitric oxide (NO) production in human gingival fibroblasts after 24 h exposure to material eluates ( n = 9). ( b ) Mean Griess assay absorbance values of human gingival fibroblasts after 24 h of exposure to material eluates.

    Article Snippet: The commercially available human gingival fibroblasts (hGF) cell line (CLS Cell Lines Service GmbH, Eppelheim, Germany; catalog number 300703) was used for this study.

    Techniques: Griess Assay

    ( a ) Boxplot of LDH release after 24 h direct contact of human gingival fibroblasts with the tested denture base materials ( n = 9). * p < 0.05. ( b ) Mean LDH assay of human gingival fibroblasts after 24 h of direct contact with the tested denture base materials.

    Journal: Journal of Functional Biomaterials

    Article Title: Evaluation of Post-Processing Time’s Influence on Biocompatibility of 3D-Printed Denture Base Resins

    doi: 10.3390/jfb17040188

    Figure Lengend Snippet: ( a ) Boxplot of LDH release after 24 h direct contact of human gingival fibroblasts with the tested denture base materials ( n = 9). * p < 0.05. ( b ) Mean LDH assay of human gingival fibroblasts after 24 h of direct contact with the tested denture base materials.

    Article Snippet: The commercially available human gingival fibroblasts (hGF) cell line (CLS Cell Lines Service GmbH, Eppelheim, Germany; catalog number 300703) was used for this study.

    Techniques: Lactate Dehydrogenase Assay

    ( a ) Boxplot of LDH release in human gingival fibroblasts after 24 h exposure to material eluates ( n = 9). * p < 0.05. ( b ) Mean LDH assay absorbance values of human gingival fibroblasts after 24 h of exposure to material eluates.

    Journal: Journal of Functional Biomaterials

    Article Title: Evaluation of Post-Processing Time’s Influence on Biocompatibility of 3D-Printed Denture Base Resins

    doi: 10.3390/jfb17040188

    Figure Lengend Snippet: ( a ) Boxplot of LDH release in human gingival fibroblasts after 24 h exposure to material eluates ( n = 9). * p < 0.05. ( b ) Mean LDH assay absorbance values of human gingival fibroblasts after 24 h of exposure to material eluates.

    Article Snippet: The commercially available human gingival fibroblasts (hGF) cell line (CLS Cell Lines Service GmbH, Eppelheim, Germany; catalog number 300703) was used for this study.

    Techniques: Lactate Dehydrogenase Assay

    ( a ) Boxplot of caspase-3/7 activity in human gingival fibroblasts after 24 h of direct contact with the tested denture base materials ( n = 18). * p < 0.05. ( b ) Mean caspase-3/7 activity of human gingival fibroblasts after 24 h of direct contact with the tested denture base materials.

    Journal: Journal of Functional Biomaterials

    Article Title: Evaluation of Post-Processing Time’s Influence on Biocompatibility of 3D-Printed Denture Base Resins

    doi: 10.3390/jfb17040188

    Figure Lengend Snippet: ( a ) Boxplot of caspase-3/7 activity in human gingival fibroblasts after 24 h of direct contact with the tested denture base materials ( n = 18). * p < 0.05. ( b ) Mean caspase-3/7 activity of human gingival fibroblasts after 24 h of direct contact with the tested denture base materials.

    Article Snippet: The commercially available human gingival fibroblasts (hGF) cell line (CLS Cell Lines Service GmbH, Eppelheim, Germany; catalog number 300703) was used for this study.

    Techniques: Activity Assay

    Hierarchically clustered heatmap summarizing the biological responses of human gingival fibroblasts to the tested denture base materials.

    Journal: Journal of Functional Biomaterials

    Article Title: Evaluation of Post-Processing Time’s Influence on Biocompatibility of 3D-Printed Denture Base Resins

    doi: 10.3390/jfb17040188

    Figure Lengend Snippet: Hierarchically clustered heatmap summarizing the biological responses of human gingival fibroblasts to the tested denture base materials.

    Article Snippet: The commercially available human gingival fibroblasts (hGF) cell line (CLS Cell Lines Service GmbH, Eppelheim, Germany; catalog number 300703) was used for this study.

    Techniques:

    Effect of different concentrations of L-PRF on gingival fibroblasts viability. Higher concentrations of L-PRF resulted in statistically higher cell viability compared to the control group. This positive impact diminished in concentrations lower than 25%. Error bars are indicative of standard deviation.

    Journal: Scientific Reports

    Article Title: Effect of simultaneous application of low-level diode laser and leukocyte platelet-rich fibrin on gingival tissue healing: an in vitro investigation

    doi: 10.1038/s41598-026-38012-w

    Figure Lengend Snippet: Effect of different concentrations of L-PRF on gingival fibroblasts viability. Higher concentrations of L-PRF resulted in statistically higher cell viability compared to the control group. This positive impact diminished in concentrations lower than 25%. Error bars are indicative of standard deviation.

    Article Snippet: Standard human gingival fibroblast cell line (HGFs, NCBI: C165) was obtained (Pasteur Institute, Tehran, Iran), defrosted, and cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco, USA) containing 10% fetal bovine serum (FBS, Gibco, USA) and 1% antibiotics (streptomycin/penicillin, Gibco, USA) under 5% Co2 and 37°C in 95% humidity.

    Techniques: Control, Standard Deviation

    Effect of L-PRF and diode laser on gingival fibroblast viability through MTT assay. Data is reported as the mean of cell viability (percentage) and error bars are indicative of standard deviation. Simultaneous application of L-PRF and laser diode resulted in the highest statistically significant cell viability compared to other study groups ( p < 0.01).

    Journal: Scientific Reports

    Article Title: Effect of simultaneous application of low-level diode laser and leukocyte platelet-rich fibrin on gingival tissue healing: an in vitro investigation

    doi: 10.1038/s41598-026-38012-w

    Figure Lengend Snippet: Effect of L-PRF and diode laser on gingival fibroblast viability through MTT assay. Data is reported as the mean of cell viability (percentage) and error bars are indicative of standard deviation. Simultaneous application of L-PRF and laser diode resulted in the highest statistically significant cell viability compared to other study groups ( p < 0.01).

    Article Snippet: Standard human gingival fibroblast cell line (HGFs, NCBI: C165) was obtained (Pasteur Institute, Tehran, Iran), defrosted, and cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco, USA) containing 10% fetal bovine serum (FBS, Gibco, USA) and 1% antibiotics (streptomycin/penicillin, Gibco, USA) under 5% Co2 and 37°C in 95% humidity.

    Techniques: MTT Assay, Standard Deviation

    Effect of L-PRF and diode laser on gingival fibroblast proliferation through Flowcytometry. The data is reported as the number of cells/5 μL and error bars are indicative of standard deviation. Simultaneous application of L-PRF and laser diode resulted in the highest statistically significant cell count compared to other study groups ( p < 0.01).

    Journal: Scientific Reports

    Article Title: Effect of simultaneous application of low-level diode laser and leukocyte platelet-rich fibrin on gingival tissue healing: an in vitro investigation

    doi: 10.1038/s41598-026-38012-w

    Figure Lengend Snippet: Effect of L-PRF and diode laser on gingival fibroblast proliferation through Flowcytometry. The data is reported as the number of cells/5 μL and error bars are indicative of standard deviation. Simultaneous application of L-PRF and laser diode resulted in the highest statistically significant cell count compared to other study groups ( p < 0.01).

    Article Snippet: Standard human gingival fibroblast cell line (HGFs, NCBI: C165) was obtained (Pasteur Institute, Tehran, Iran), defrosted, and cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco, USA) containing 10% fetal bovine serum (FBS, Gibco, USA) and 1% antibiotics (streptomycin/penicillin, Gibco, USA) under 5% Co2 and 37°C in 95% humidity.

    Techniques: Standard Deviation, Cell Characterization